How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

Do you know what happen in reaction between Ni2SO4.6H2O and DMG (dimethylgolyoxin)?

I want to use DMG as a reagent for detecting Ni in a solution. in their reaction they form a complex together which color is pink. I attached a picture of the reaction. my problem is I can`t find...

01 January 2021 7,200 3 View

No title

This phase is a ternary phase in Al-rich region of Al-Ni-Cr alloy system with a typical chemical composition of Al74Ni8Cr18.

06 September 2020 5,950 3 View

How can i make a 1000 ppm Ni(II) solution?

I try to prepare 100ml of a 1000 ppm Ni(II) solution by solving NiSO4.6H2O in ionized water. To do so I add 0.45g of NiSo4.6H2O to the water. i would appreciate if anyone tell me whether it is...

31 August 2020 7,807 4 View

Is it possible to see the redox peak in CV while did not observe plateaus in the charge-discharge curves?

Dear researchers I see clear redox peaks in CV, but i did not plateau in the charge-discharge curves. Does anyone know the reason?

04 February 2020 707 9 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

How can i amplify fragment of approximately 3100 bp?

Hi, I want to amplify fragment of approximately 3100 bp from bacterial genome for cloning in pET28a using NcoI and XhoI restriction enzymes. I performed PCR using following program:...

24 July 2019 6,115 7 View

Is there any procedure to synthesis [(NH3)5 Ru SC6H5]2+ from RuCl3 and Diphenyl disulfide?

I need to prepare [(NH3)5 Ru SC6H5]2+ from RuCl3 and Diphenyl disulfide. So at first Ru(III) should be reduced to Ru(II) by zinc powder and then Ru(II) oxidize to Ru(III) by diphenyl disulfide to...

19 May 2019 1,064 1 View

Gold nanoparticle deposited on carbon printed electrode generate redox peak in the CV?

When i test the gold nanoparticle coated carbon printed electrode in the PBS, I see the redox peaks but the potential for the Au to reduce or oxidise is not in the range of my CV(-0.3 to 0.7). Can...

27 March 2019 7,519 3 View

What are the best compounds to increase neuronal proliferation?

I am working with Parkinson cell lines ( SH-SY5Y ) and stem cells, I have to find compounds that increase proliferation of neurons. Thank you

29 January 2019 539 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View