What are the alternative techniques that can be used in place of northern blotting?

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What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Does plant based medicine have less side effects?

I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...

08 July 2024 9,935 10 View

Hey everyone. I am facing some problems while transfecting A172 cell line with three plasmids for Dual Luciferase Assay. Any suggestions?

(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).

16 June 2024 6,687 2 View

Are the frequencies extracted from SdH oscillations nearly the same for different cemical substituted samples?

Generally, the chemical substitution should adjust the Fermi level, and the computed TSS indeed suggests that the Fermi pocket for the three samples different, so why are the frequencies extracted...

17 May 2024 1,897 1 View

Computed TSS seems to suggest that the sample should have stronger sdH oscillations, whereas experimentally extracted SdH oscillation is weak?

The sample nature is thin film

17 May 2024 8,459 0 View

How to define t shape gate structure in silvaco atlas?

I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0.03...

13 May 2024 3,059 0 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

How to define t shape gate structure in silvaco atlas?

I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0...

13 May 2024 5,026 0 View

When can the fitting of the fluorescence anisotropy decays have the negative component ?

it would be helpful if you provide the reference to your answer

11 May 2024 5,989 4 View

What concentration of Nystatin should be used in Nutrient Agar medium?

I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...

21 April 2024 6,039 5 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How can one determine the conversion factor for a radiolabeled substrate to a radiolabeled product using slopes from linear regression equation?

I am preparing a radiolabeled product using a radiolabeled substrate. I have the cpm values for different dilutions of both my known substrate and unknown product ? How can one determine the...

27 July 2024 3,604 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View