Articles invited for Natural Science Refresher, January Issue 2018. Does someone want to collaborate with us?

Dear Sir/Madam, Wishing you all a very happy and prosperous new year 2018 It is my proud privilege to inform that we are going to publish our monthly science magazine "Natural Science Refresher...

31 December 2017 6,524 0 View

How climate change affects the biological characteristics of a plant?

the biological characteristics like growth, physiology, cytology, phenology etc.

06 July 2016 8,854 4 View

What are the traditional uses of medicinal plant?

Please provide the list of some medicinal plants used by you and in your locality. for example: 1. Azardica indica = skin treatment, blood purification. Looking for your positive responses.

05 June 2016 1,397 5 View

How much change have you observed in the weather system of your locality?

Every place have a particular weather pattern at a particular time. The climate change may have the effect on this weather pattern. So I want to know about what changes (like change in...

05 June 2016 5,718 4 View

How we can check and study the effect of pollutants on plant leaf cells?

05 June 2016 5,417 4 View

What are the principals of computational chemistry research?

what are the tools and techniques used in computational chemistry research? Please give some suggestions. Thanks!

31 December 2015 4,778 3 View

What are the important points a researcher should focus to write a review paper?

11 December 2015 9,505 3 View

Is weather effects the chemical constituent of a plant?

Is weather effects the chemical constituent of a plant? if yes, then which weather is suitable for extraction of phytoconstituents.

04 May 2015 8,673 1 View

What are the best methods to store the plant samples for long use?

03 April 2015 8,161 6 View

Do you know about synthetic milk?

Do you know about synthetic milk? If yes what chemicals are used to prepare it? what are the adverse effects of synthetic milk on health? how we can differentiate between natural milk and...

02 March 2015 9,116 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View