What statistical method can I use to evaluate stress levels on individuals who consume red and black rice? Would 10 individuals be sufficient ?

I am in my master's thesis and I am seeking to look at stress levels on individuals who consume red and black rice. Is it enough to observe 10 individuals? What statistical method should I use?

31 December 2018 3,462 5 View

Why do you use Ferric Reducing Antioxidant Power and Metal Chelating Activity in identifying antioxidant in plant sample (Besides the obvious answer)?

I want to know more about the link between Ferric Reducing Antioxidant Power and Metal Chelating Activity in antioxidants. I need to know the chemical underpinnings behind using these two methods....

09 October 2018 8,296 4 View

Can you measure HbA1c in less than 3 months for mice since they have smaller blood volume?

I'm starting a research on mice to measure HbA1c levels and would want to get results in a few weeks (6-10weeks).

07 August 2018 1,176 8 View

How does IDP (intrinsically disordered protein) fail to conform to the lock and key model?

Protein-substrate interaction

03 April 2016 9,841 0 View

What happens to bacterial membrane at 45C?

02 March 2016 9,388 6 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View