Hello dear,

You can evaluate in vitro alpha-glucosidase inhibition assay according to method of Matsui et al., 2007.

Materials and reagents for assay-

1. Alpha-glucosidase enzyme.

2. Substrate: Sucrose.

3. Positive control: Acarbose.

4. Sodium dihydrogen orthophosphate (NaH2PO4.2H2O).

5. Disodium hydrogen phosphate (Na2HPO4.2H2O).

6. Glucose reagent.

7. Working solution (Phosphate Buffer; 80 mM, pH 7.0, 25 oC)

Best of Luck

Dharmendra Arya