I plan to look at T cells from muscle tissue using flow cytometry.
I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...
27 June 2024 9,627 1 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....
17 June 2024 8,956 5 View
can someone use quantum mechanics to explain the intuition behind this phenomenon?
07 June 2024 9,584 2 View
Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...
07 June 2024 8,723 2 View
to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?
06 June 2024 5,095 1 View
Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...
31 May 2024 4,613 3 View
I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...
28 May 2024 4,068 2 View
I am working on vehicle pedestrian safety at 4-way intersections with traffic signal control lights. I need dataset available for Kaduna metropolis busy 4-way intersection with pedestrian count...
21 May 2024 4,872 1 View
Where can I find Business and Professional Communication Quarterly's manuscript acceptance rate?
18 May 2024 1,303 1 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?
02 July 2024 8,261 0 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
social factors that influence white-collar crime
22 June 2024 9,121 3 View
Does anyoane have experience with angiogenesis assay in 3D matrix derived from egg white?
03 June 2024 522 0 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View
Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?
26 May 2024 8,124 0 View
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
23 May 2024 3,356 1 View
Hi All, Our group is doing some flow cytometry assays using an HLA-A*0201 tetramer presenting an HBV peptide (FLLTRILTI) and we're getting some strange results... We ran tetramer flow on a bunch...
16 May 2024 8,123 1 View
I am evaluating histological slides (H&E) of brown adipose tissue from rats. And counting the number of unilocular adipocytes to evaluate the whitening process. How to identify? Would it...
13 May 2024 7,876 0 View