There are studies that has examined persistence and decay of bacterial pathogens, fecal indicator bacteria ( FIB ) and emerging real- time quantitative PCR ( pPCR ) genetic markers for rapid detection of fecal pollution in manure- amended agricultural soils. .....Potential diferences in the decay of these genetic markers could lead to different risk assessment The most important aspect in microbial inactivation is to secure food safety by reducing the number of spoilage bacteria and also eliminating pathogenic bacteria in foods. The most common technique for microbial inactivation is presently thermal processing. For more information consult eprints.lib.hokudai.ac.jp
Using methylene blue or any indicator in new enrichment medium and inoculated with washed cell and examination under microscope. The dead cells became blue color meanwhile the inhibited cells became active and absorbed the pigment.
Decomposition or rotting, the breakdown of organic matter usually by bacterial action, resulting in the formation of other substances of less complex constitution with the evolution of ammonia or its derivatives and hydrogen sulfide; characterized usually by the presence of toxic or malodorous products.