I wish to evaluate acquired immune response in the brains of rodents by immunohistochemistry, focusing in cytotoxic T cell and effector B cells/plasma cells can anyone help me out in choosing which markers I can use? Thank you in advance.
Luis
Hello Luis,
Usually you could use CD8 for cytotoxic t cells (CTL) to identify them and Granulysin/Perforin to evaluate their activity. For B cells you should probably use CD19 and/or CD20. Also another marker of B cell activation is CD27. Usually you should not be able to find B cellsin the brain and even less likely is finding plasma cells in the brain since these are usually in the germinal centers in the lymph nodes or in bone marrow. However in several models of encephalytis TH1 CD4 cells are present in the lesions, so, maybe you should consider evaluating CD4`s too, i think its more likely that you find these ( and if you stain for citokines such as IFN-gamma, IL-4 and IL17a you could be able to determine if these are TH1, Th2 and TH17 respectively).
Best regards
Thank you Julio for your help. I will look into the markers you advised.
I want to evaluate immune response agains a viral vector transduction to evaluate vector toxicity in the brain. I wish to evaluate innate immunity but also adaptive immunity.
ATB
Luis
Are the B cells from mice? You can add CD138, but I need to know from what specie your cells are to answer.
Yes, if you are looking at mouse cells, you should look at CD138 for plasma cell. If looking at human plasma cell, you might add CD38 instead.
Hi,
Thank you for the answers. I will be doing the study in rats as I will be analysing the brains in the context of parkinson disease modes.
Thank you once again
Luis
Hi Luis -
We also use CD138 for plasma cells (in human tissue), but I also wanted to add a small addendum to Dr. Delagados answer (as I understand it) - if you are specifically looking for plasma cells - CD20 is not appropriate as it is down-regulated upon terminal differentiation of B cells to plasma cells; but I am grateful for his tip about granulosin and perforin for CD8 activity - so thank you!
Best wishes
Pia