What to do if i am getting very light bands on SDS-PAGE ?

I didnt add any molecular marker in sample. moreover light bands are seen. My protein is showing proteolytic activity and fibrinolytic activity too. But bands are very light . please tell me...

16 August 2019 7,779 6 View

Which algorithm might be worth trying for this dataset ?

Please find the sample dataset attached. Here, as we can observe there is a forecast and actual data. However, we have only for about 8 years (2000- 2008) of data. Trying: XGBoost, ARIMA...

07 August 2019 8,765 3 View

Let's say that I have a plot of Biomass concentration v/s time. How do I determine the specific growth rate curve?

I am fairly new to the field of Biotechnology.

19 February 2018 5,936 5 View

Which are the pseudo-elastic or highly elastic (yet economical) metals except for Shape memory metals ?

I am looking for metal sheets that are very elastic and yet do not lose it fast.

19 December 2017 7,253 1 View

Could you please suggest me an alternative Calibration method?

-I want to calibrate an economical self-designed four-point bending setup. (Please refer to the image) -The plan is to test specimens on my setup and the standard Bending setup with specimen...

18 April 2016 1,983 10 View

What are the best markers to distinguish between pericyte and smooth muscle cells?

I am working on generating pericytes and SMCs but since both have common origin it is difficult to distinguish between the two lineage best on markers. Does anyone know of very specific markers...

17 May 2013 5,297 15 View

Can someone kindly help me with a simple laboratory procedure for Detection of viability of spores of LAB?

For my research on formulation of fermented product from vegetable agro-wastes i wish to check for the viability of spores of LAB to create a probiotic soup mix.

06 December 2011 3,987 1 View

How to interpret the linear regression coefficients ?

We are trying to understand the impact of number of workdays on sales. Please find reprex below: ``` library(tidyverse) # Work days for January from 2010 - 2018 data = data.frame(work_days =...

01 January 1970 6,614 8 View

Which regression algorithm could be applied for correcting measured values?

Please consider the sample dataset below. In simple terms, An sensor is defective and hence measurement has incorrect values since 2000 and we have the data for 10 years with both: measured and...

01 January 1970 4,812 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View