What analytical tool I should use to quantify presence of silane coated on pdms surface ?

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What's the best method to separate Nanoparticles conjugated to antibodies from unbounded antibodies remaining in the solution?

Hey all, I'm working on synthesizing Gold nanoparticles (GNP) - antibodies conjugates for biomedical purposes. We are struggling to find an efficient method to sperate the unconjugated antibodies...

03 March 2022 8,959 4 View

Does anybody has the biodot zx1010 manual?

Hello! i´m trying to use the biodot zx1010 to make the test and control line on a lateral flow strip but the manual that came to our machine it isn´t the right one, and doesn´t have much...

25 January 2021 6,790 1 View

How do I make a mountage of separate channels and merged image in Fiji/ImageJ?

I have images with different channels, I would like to make a mountage of the single channels+the image resulting from the merging of them. in the attached images you can see the different...

06 August 2019 1,814 3 View

TMAH etch-Hotplate temperature issues- anyone had a problem of hotplate temperature falling down/not reaching your set point ?

Hot plate temperature falling down/doesn't rise beyond a point. I'm running a TMAH etch of silicon in a 2000mL beaker with wafer in a teflon holder. The hotplate is set at 92C with the external...

03 October 2017 8,730 0 View

What ALE parmameter values should I take to avoid excessive distortion during cutting simulation in ABAQUS/EXPLICIT?

While cutting simulation the process is getting aborted due to excessive distortion of the mesh . What ALE frequency and remeshing sweeps must I consider to avoid this . I am using J-C model and...

01 November 2016 7,417 7 View

How can I eliminate back flow & air bubbles in micro-channels of pdms microfluidic device ?

I have a PDMS microfluidic device with one channel (10mm X 5mmX 50 um) having an inlet (2mm diameter hole) and outlet (2mm diameter hole). I am trying to pass solutions through inlet and pulling...

21 April 2016 9,251 4 View

How do I remove PDMS mould without it sticking to the silicon master and without damaging the imprint on PDMS?

Also how to clean the silicon master after the pdms has been removed? PDMS residue is left behind.

20 April 2015 7,067 12 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

Why specific capacitance is not proportional with the specific surface area?

Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...

26 July 2024 9,156 2 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View