Hello everyone,

I intend to detect genetic mutation using Single-Strand Conformational Polymorphism and the loading dye components from Cold Spring Harbor Protocols is listed below:

95% (v/v) formamide

10 mM NaOH

0.25% (w/v) bromophenol blue

0.25% (w/v) xylene cyanol

Unfortunately, I don't have formamide. Anyone has an experience working with an alternative?

I will be grateful with your answers.

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