Hello everyone,
I intend to detect genetic mutation using Single-Strand Conformational Polymorphism and the loading dye components from Cold Spring Harbor Protocols is listed below:
95% (v/v) formamide
10 mM NaOH
0.25% (w/v) bromophenol blue
0.25% (w/v) xylene cyanol
Unfortunately, I don't have formamide. Anyone has an experience working with an alternative?
I will be grateful with your answers.