What add trichloroacetic acid in MTT method for cancer cell assay ?
I believe you are asking why TCA is added in a MTT assay? If yes, it is used to precipitate proteins.
lower concentrations from plants essential oil are higher inhibition, Why?
10 November 2016 9,600 0 View
This is pictures for kind of plant was did by Transmission electron microscope JEM2100
07 August 2016 9,438 0 View
Can anyone explain the pictures? please open the attachment file what is the deference between these pictures one did by Scanning electron microscopic (A) and another did by Transmission electron...
07 August 2016 8,833 3 View
How to be determination of bioactive compounds in aromatic plants ?
11 December 2014 8,709 6 View
Is cancer cells (MCF-7) purchased as ready from research institutes be harmful to human body?
11 December 2014 8,411 2 View
Is cancer cells (MCF-7) purchased as ready from research institutes be toxic to the human body?
11 December 2014 5,698 0 View
What type of cells can be measured using Cell Counting Kit-8?
11 December 2014 1,947 0 View
Why is extraction with methanol the best from ethanol?
11 December 2014 3,195 2 View
How long of a pre-incubation time is required prior to the assay?
11 December 2014 8,078 0 View
Why add penicillin or other antibiotics when culture or keeping cancer cells? Why keep cancer cells in atmosphere of 5% CO2?
11 December 2014 7,629 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View