Hey guys,

i'm looking for a general answer. My actual lab is blocking their WB with 5% Milk in TBST and then using 0,5% for the 1. and 2. antibody solution. 

My last lab was using 5% milk for blocking and antibody incubation. 

How much milk do you use and WHY?

I can't find out the reason my lab mates reduce milk for antibody incubation.

Best,

Konstantin

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