Konstantin Bagnjuk

4 Questions 17 Answers 0 Followers

Questions related from Konstantin Bagnjuk

Cell culture and Ceramides: How to evade the solution problem of C16 and C2 Ceramides?

Dear Community, as i want to find out the impact of ceramide on my cells in DMEM-F12 medium. I dissolved C16-CER in Ethanol and added it to my medium. After having a look through the microscope, i...

03 March 2017 1,124 2 View

Western Blot - How much dry milk in the antibody solution?

Hey guys, i'm looking for a general answer. My actual lab is blocking their WB with 5% Milk in TBST and then using 0,5% for the 1. and 2. antibody solution. My last lab was using 5% milk for...

10 October 2016 8,537 13 View

Has anybody an idea how to evaluate confluency?

I'am looking for a method to examine confluency of cells in pictures taken by a microscope-camera application (sample picture is attached). It would be very nice if you could help me with a method...

08 August 2016 9,591 13 View

Confluency determination with human granulosa cells?

Dear community, i'm looking for a possibility to determine the confluency of granulosa cells in a dish. We stimulated cells with Pam3Cys-Ser-(Lys)4 (a TLR2 agonist) and observed them under the...

07 July 2016 6,297 2 View