Hi,
I am using a closed acryl-glass chamber for plant-labelling experiments (12C & 13C). We want to measure 13CO2 and δ13C using Picarro G1101-i. When we tested the air-tight chamber with a plant inside, we got a sudden increase in δ13C (+20) and slowly decreased to normal values (-14) with in an hour. But when we did a backgroud check for the chamber alone without any plant the δ13C values were extremely high (+80 to +150) without any labelling and with new chambers.
Do any one have experiences with Picarro and δ13C weird values in a closed system?
Is there any material (Acryl glass, steel values, butyl rubber) influencing these values?
Let me know if any one can help me to solve this problem.
Thanks alot in advance.
Best regards,
Saranya
Hi,
I have solved the above mentioned problem with the help of my collegues, we washed the chambers (as they were new) and left the chambers outside overnight with water. This process helped us to remove the gases and chemicals from the Acryl glass, silicon etc. Today, we measured the δ13C with Picarro, they were in good range (-20 to -15).
I hope this answer could help someone who has weird values.
Best regards,
Saranya
Dear Saranya
I think the links below will benefit you in your search
Article Use of δ13C, δ15N, and carbon to nitrogen ratios to evaluate...
https://www.esrl.noaa.gov/gmd/outreach/isotopes/deltavalues.html
https://www.science.gov/topicpages/0-9/13c+depleted+co2
https://www.ethz.ch/content/dam/ethz/special-interest/usys/ias/grassland-sciences-dam/documents/Research/COST_Action_ES0806_SIBAE.pdf
Best Regards
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