Dear all,
We are trying to investigate the effect of chronic exposure of a specific drug on mice. The NPS will be given chronically.
I have found in literature:
In Expose System from Scirec and Inhalation Tower from Buxco.
Has anyone experience with any of these systems with pros and cons?
What is really interesting for me is the type of restrainer Allay vs. Net and the fact if the mice are allowed to breathe normally or not for animal welfare reasons.
A response would be greatly appreciated.
Thanks.
Best Regards,
SG
You may pl. contact Dr. Steve Cape at University of Colorado, Boulder. I believe, he will be able to throw some light on your query.
Regards.
Dear Stefano, I recently looked into inhalation systems. You can use a chamber where mice move freely and breath the vapor or suspension but it is very hard to control the dosage. I found the PennCentury system looking online for intratraqueal delivery. It is a more difficult technique (they can help with training) but you will have perfect dosage control. I hope this helps. Dani.
Dear Daniela,
indeed I have considered a whole body system because the animals are more free and you could provide NPS such in the humans. I assume that intratracheal would not have the same distribuition as given via aerosol. Additionally, in the WBP chamber some behavior could be conducted such freezing responses and increase in respiratory rate could be assessed as measure of fear.
What do you think since you long experience also with Dr. Hen and other behavioral groups?
Thanks,
Stefano