Our goal is to decrease the quantification limit as much as we can. We have a HClass-Xevo TQD Waters with a BEH C18 column 1.7 100 mm
simple scheme in our lab if we would like to decrese LOQ without much change in method:
extraction step:
increase extraction volume of plasma
increase volume of extraction solution or extract twice
use SPE
combination above methods
detection and quantification:
increase/decrease injection volume - LC step
increase/decrease injection volume - MS step (change split, change capillary distance etc)
other possible- change method at all - play with conditions, play with different m/z, different mobile phase, column . it is a huge field to play on.
SPE, as noted by Daria, or SPME. SPME will give you a significant concentrative effect.
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