We are looking for a system to test how our designed polymer affects cytokines in the human body? Do you have any suggestions for a system to do so?

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Only middle wells of iPSC differentiate into cardiomyocytes?

Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...

02 May 2024 6,314 1 View

A260/230 ratio issue when RNA isolation from primary cell?

Hello Currently, we are separating primary cells from mouse spleen or thymus into single cells, and then diluting them by 1/10 from 10^7 cells by cell count to see which line mRNA can be...

20 March 2024 5,467 0 View

Why won't my induced pluripotent stem cells form a pellet when passaging?

I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...

11 June 2023 5,454 1 View

Why don’t my iPSC form a cell pellet when centrifuged?

I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...

10 June 2023 8,814 1 View

How to keep AZ-P4620 from making bubbles?

Hello dear research community, recently I'm struggling with my photoresist P4620 which makes bubble under its polymer substrate whenever exposed to UV. I know it has degassing problems when...

06 December 2022 2,135 0 View

Sample power calculation for path analysis?

Can an SEM sample size calculator calculate a sample power for path analysis? If so, how do I use it? What number should I put in the latent and observed variable slot? For example, 6 continuous...

27 November 2022 5,916 2 View

Loss of confluency during iPSC culture?

I am culturing human induced pluripotent stem cells for differentiation into cardiomyocytes via the Wnti-directed protocol attached here. After passaging into a 12 well plate to start the...

22 February 2022 7,405 2 View

RNA extraction from blueberry fruit?

Hi RNA experts, I am trying to extract RNA from my blueberry fruit samples. They have been stored in -20 C for one or two months and then they were moved to -80 C. I used the Qiagen RNeasy Plant...

10 November 2021 168 6 View

How to get an appropriate Fleiss kappa sample size?

Hello, I`d love to ask you for a question regarding appropriate sample size of interobserver reliability study, I`ve working on a study that evaluate a reliability of a new classification of...

24 September 2021 4,408 3 View

Protocol for administration of acetycholine and norepinherphrine to cells in in vitro culture?

Hello all! I work with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for my research. I am seeking to administer the positive chronotrope epinephrine and negative chronotrope...

29 June 2021 6,568 0 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View