Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

Does color or lighting/reflection influence behavioral test in mice?

Our pole test apparatus includes a metal pole covered with white cotton thread, with a grey plastic base that gives a slight reflection. In our experiment, many mice reach within a few centimeters...

17 January 2020 9,969 1 View

Is there any atmosphere that Titanium chloride can make fume?

I use TiCl4 to check if there's water in the glovebox. when I opened TiCl4 there were a lot of fumes, but I never guess where the water comes. Because there was no oxygen inside. So I didn't sure...

13 January 2020 9,127 1 View

Is there a perfect purification method for semiconductor precursor?

I tried synthesize Ethyl derivated cyclopentadienyl tris(dimethylamido)Zirconium But I have some problem in purification process I want to remove all the solvent from product (toluene) but it...

10 June 2019 4,889 2 View

Why is the PL peak blue shifted?

The PL peak for bulk 3C-SiC is 2.3eV approximately. But the peak for Polycrystalline SiC(bulk) sample is observed to be near 2.8eV. So a shift is observed. I am interested to know the reason...

06 April 2017 2,018 3 View

Does ellagic acid present with lung, liver, intestinal toxicity?

09 December 2015 5,320 3 View

How do you gate naive T cell population?

Hi I was wondering if anyone can shed some light on this problem I'm stuck with. (Please check the attached PDF file) [Background] I isolated naive T cell (or what I thought to be) using a...

26 July 2015 5,021 4 View

Can you identify this insect?

01 February 2015 6,391 8 View

Methods/ Software to determine sun position at my latitude

I have a ground based backscatter lidar, operating at vertical (90 degree zenith) everyday during day time. The Lidar manufacturer advise us not to operate it when the sun disc enter our Lidar's...

11 July 2013 4,169 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View