Wastewater treatment and PCR tech ?

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Drinking water quality evaluation?

Dear professors which test is more important in evaluation of water quality for drinking use: 1. Total plate count? or 2. Total Coliform ?

06 July 2018 3,308 9 View

Environmental journals for free publishing?

Dear professors I am asking about environmental journals for free publishing.

05 June 2018 841 5 View

What we mesure to study climate change in Iraq?

We are planning to start complet study about climate change in Iraq, weather stations shows great variation of temperatures degrees during the last decades. My question about the basic steps to...

05 June 2018 2,980 16 View

Does anyone have suggestions on how to extracts heavy metals from plant tissue?

Dear professors i am asking about the extraction of heavy metals from plant tissue which use in bioremediation of polluted soils by HM ? also we are preparing fungal culture to assess its ability...

05 June 2018 1,749 6 View

How can extract heavy metals from fungal biomass ?

three species of fungi treated with different heavy metal concentrations.

04 May 2018 7,438 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Maurice Ekpenyong

Applications of PCR technology in wastewater treatment have been copiously reported. See the link and attached documents. I am sure there are very many more. Article Quantitative real-time PCR approaches for microbial communit...

Stephen Reynolds

Havn't been in a microbiology lab in some time but you could pair culturing techniques of a waste water sample with follow up PCR on pure colonies for identification if you have trouble with sensitivity.

Ahmed Housanie Elsappah

Hi, Dr. Mazin N Fadhel

1- PCR AND THE DETECTION OF MICROBIAL PATHOGENS IN WATER AND WASTEWATER

https://pdfs.semanticscholar.org/aaf3/6a6f0855c8a6f13c794a889df08ecaddadec.pdf

2- Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: Applications and considerations

Article Quantitative real-time PCR approaches for microbial communit...

3- Host range of antibiotic resistance genes in wastewater treatment plant influent and effluent

Article Host range of antibiotic resistance genes in wastewater trea...