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03 March 2021 5,360 2 View

Why are spectrophotometric absorbance values much higher than the initial value after electrochemical oxidation of organic matter?

After electrooxidation of an organic material, I get an excessive increase in absorbance values in wavelength scanning. What could be the reason for this? Can Radical formation cause...

20 January 2021 3,156 3 View

Global change in protein expression?

Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...

03 January 2021 1,388 8 View

Tetracycline/doxcycline treatment?

Hi all, I am not sure whether or not this question was asked. I wonder can I induce expression of my protein with tetracycline or doxycyxline while seeding cells instead of one day after seeding...

05 November 2020 2,417 4 View

AMD in NAMD or flooding in GROMACS?

Which software would you recommend for aMD? Gromacs or NAMD? Also, what do you think about Gaussian aMD implementation in NAMD? Is it preferable to others?

29 October 2019 2,981 2 View

How to do Bland Altman Plot in measurement pairs in SPSS or Excel?

Hi all, Although I watched all the videos about Bland Altman plot in SPSS, I could not find any information regarding how to use this statistics in multiple replicates for each method. Hope...

13 September 2018 9,359 14 View

Calcium Phosphate Transfection Problem?

Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...

23 March 2018 5,856 7 View

What would be the cause of high density growth in 1/1000 and 1/10.000 diluted LB Agar?

Dear all, We cultivated E. Coli in LB Agar in 6 plate which contain respectively sample itself, 1/10 diluted sample, 1/100, 1/1.000 and 1/10.000 . Later we incubated plates at 37oC for 24h. When...

24 February 2018 5,372 3 View

How to find in ANSYS ALPD and BETD value?

Hello everyone, I am using ansys but I am somewhere I have calculated ALPAD and BETAD values but I want to enter ALPD and BETD values in its material. how to find these values in ANSYS.Thanks in...

21 January 2018 8,584 1 View

SiRNA Transfection to COLO205, SW620, HT29?

Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...

04 December 2017 2,369 3 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View