Virus eater! - FAQS.TIPS

More Shreyans Chordia's questions See All

Can you suggest inexpensive methods to quantify ATP from fungi in liquid cultures?

Preferably non-enzymatic methods as I cant access a luminometer.

04 May 2013 2,603 0 View

Can anyone suggest good intron prediction software?

Is intron site prediction reliable?

09 October 2012 2,179 3 View

Kindly explain this aberrant result of RNA isolation from Tumor cells.

Please correct me if I am wrong- Total RNA extracted from eukaryotic cells has three distinct visible bands on an agarose gel- 28SrRNA, 18SrRNA and the least prominent 5SrRNA & 5.8SrRNA. And...

02 March 2012 2,843 3 View

dG value of Primers

This a self annealing result of an upper primer from OLIGO EXPLORER 5'-CATCGCACGTCGTCTTTC-3' : : :

01 February 2012 364 16 View

Effecting of Ramping on PCR

I came across these terms in a post on a topic here: Resistant effect - During Increase of temperature Pelletier effect - During Decrease of temperature Can anyone please explain...

01 February 2012 1,866 0 View

Genetic changes during differentiation

What are the genetic changes that happen in a cell undergoing differentiation? What is our current knowledge in this area?

01 February 2012 7,638 10 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

Amarnath Pisipati

Hi Shreyas,

Really interesting. Do you have any further information on Virophage... like can we expect them to be there in humans too and unidentified till date? I am hopeful to get some information regarding these virophages.. thank you for your time.

Shreyans Chordia

Nah i havent come across any new info on virophages recently.. Virophages in humans could be a long shot though.. But i couldn predict what scientists might discover in the future..

Hey Shreyas,

We see some people in and around us who are resistant to viral infections were as we fall sick,like getting cold, fever, etc. The reason is attributed to be the strong immune system of the individuals. But, may be... or do you think if these people harbour the virophages? which we never looked into. Its just a guess. Hope we have some thing in this ..... I do not know anything. and its just a guess...

Any way nice to have a good info regarding virophages.

regards

Amar

Thats an interesting theory.. It is said our immune system in conjunction with the microbiota o our body help prevent against foreign pathogens.. These virophages could be in our microbiota..