How could we make use of novel protein sources practically in fish and animal feeding in general?

Protein is the most expensive ingredient in feed formulation and work continue to explore new and different alternative sources but do they really proved practical or advantageous?

31 December 2018 2,973 7 View

Are publication fees of some scientific journals justifiable ?

Dear colleagues, Publication fees differ widely between scientific journals but are the higher fees of some journals really justifiable in your opinion and expertise? Thanks in advance for your...

01 February 2017 7,773 5 View

How to improve the quality of higher education?

Dear RG colleagues, What are the key points that should be targeted to improve the quality of higher education in the developing countries? Thanks in advance for your responses.

31 December 2016 5,268 30 View

Improvement of IT skills in higher education ?

Dear RG colleagues, Information Technology (IT) and scientific communication skills became a necessity in modern higher education for both students and lecturers . My question is: how we can...

11 December 2016 4,296 0 View

The practical part of higher agricultural education ?

10 November 2016 7,679 4 View

The dilemma of fish meal alternatives in fish feeds ?

Researches introduce conflicting data about fish meal alternatives in fish feeds especially freshwater fish. Is there an idea, opinion, suggestion or sound knowledge about promising feedstuffs of...

10 November 2016 1,491 10 View

What are the best indicators for stress in fish?

I need expert opinions in selecting the best stress markers to use as indicators for stress in fish in natural environment and under culture conditions.

10 November 2016 6,782 22 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View