Please guide me to extract rainfall data of a particular region from IMD Grid data using Arc GIS ?

I have IMD grid data (0.25 by 0.25 resolution) in text format. How to extract rainfall data of a river basin? any tutorials? If it's not possible in ArcGIS any other software? I am familiar with...

24 August 2020 8,109 11 View

Salt Effect on Bacillus subtilis growth?

Warm greeting to all, Are there any reasons why Staphylococcus aureus more resistant against salt, if it's compared to Bacillus subtilis? and why Bacillus subtilis not growing well under salt...

11 January 2020 595 3 View

Blue coloration scattered in x-gal white/blue screening plates of Pseudomonas aeruginosa with lacZ gene ?

I want to screen a Pseudomonas aeruginosa mutant which would have a lacZ gene after being conjugated with E.coli. But unfortunately, on xgal plates i endup having accumulation of blue color...

26 November 2019 9,233 1 View

How to study changes in intermittent flows to a tropical delta using 31 year daily flow data ?

Need to understand the long term changes in the release from upstream storages to the intensively cultivated delta. Normally flows are from July to Feb months only, over the years flow has...

07 February 2018 7,072 3 View

What alteration does a coomassie brilliant blue G250 or bromophenol blue in loading buffer of SDS PAGE gels show and whether it affects quantification

We have tried running gels with both CBB G250 and bromophenol blue in separate gels and a significant variation was observed in both the gels. Bromophenol blue showed a smeared band and CBB showed...

22 June 2017 1,460 7 View

Should the element quality of all the elements be higher than a minimum or is it acceptable to have a mesh with majority of high quality elements?

Many times when a complex geometry is meshed in Ansys I am not able to get every element above a certain quality (as shown from the mesh metric data). Will such a model give an accurate result....

25 December 2016 6,733 3 View

MM function not complete in ONIOM

I have ONIOM file which consist of two layer i.e. High Layer (HL) and Low Layer (LL). HL was adjusted for 5 amino acid residues and LL for rest of residues. When I run in Gaussian09, I got failure...

01 January 1970 9,673 2 View

Error in ONIOM with variation layer of Amber in gaussian.

I had make made ONIOM input file from PDB file used 2 layers i.e. B3LYP/6-311++G(2d,2p): AMBER, but I don't know why it always get error (error message: "aborted....", "segmentation violation")....

01 January 1970 3,430 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View