What are parameters to check and compare the quality of filtering of a DSP system?

I have designed an FIR filter which discards the high frequency noise. I have tested the functionality through simulation (MATLAB). Now I wish to quantify this, that how good is my system and get...

18 January 2021 3,405 7 View

While performing DFT studies of some hetro compound in different solvents, unusual results are obtained in case of methanol. what could be the reason?

DFT Study of some heterocyclic based compound is being done in solvents of different polarity. It was observed that the potential energy barrier in methanol comes out to be exceptionally high....

15 October 2020 9,303 5 View

Can I use split basis set system in Gaussian 09 for Gold with other atoms than gold modelled at PM3 level of theory?

Can I use split basis set system in Gaussian09 for Gold with other atoms than gold modelled at PM3 level of theory? I have a system which has C, N, O, H, and Au. I want to model C,N,H and O using...

01 September 2020 7,415 4 View

What are some strategies to block primary amine surfaces in immunoassays without using any protein based blockers?

We use a primary amine surface to bind our antibodies. Thereafter we use BSA and other protein blockers for blocking. The results are not really great, and vary quite a bit on repeats. We wanted...

26 April 2020 3,101 1 View

How to find the data of a missing channel in EEG?

I don't have the data from central EEG electrodes (Fz, Cz, Pz). But data is available from other locations like F3, F4, F7, F8, FC5, FC6 etc. how can i find the data from central locations

26 December 2019 455 6 View

Why are there very few health devices based on electrochemical sensors or other simple assay technologies?

Electrochemical sensors have an inherent advantage for being simple, cheap (glucometers), especially for nucleic acid assays. Yet there are very few health devices in the market for point of care...

22 December 2019 2,599 3 View

Anybody using Ketamine in humans?

Does any body have any data on use of Ketamine infusions in human subjects for any reason? I am looking for data on response and adverse effects related with the dose of Ketamine and duration of...

19 June 2019 7,333 2 View

Is there any Ideal Soil Chart for India?

01 May 2019 6,574 5 View

What are some good methods (other than Plasma/Ozone/UV treament) for generation of reactive molecules before using APTES for coating in microchannels?

We do not have a system for oxygen plasma, corona discharge or ozone in our lab. We wanted to create reactive surface for our immunoassay on PDMS, such that it can bind to APTES. We have been...

28 April 2019 9,680 3 View

What would be the best coating buffer for covalent coating of TSH antibody (IEF Range: 5.8-6.9) on a functionalised PMMA surface?

We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer...

29 July 2018 1,144 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View