How to perform MMPBSA using Gromacs ??

Can anyone suggest me the proper way to perform MMPBSA using Gromacs ??

11 October 2020 5,203 3 View

Do all the authors of a research article receive same/ equal weightage?

The number of multiple author research articles is on the surge worldwide. Which is a great indication of collaborative research and is the way forward for almost every research field and domain....

27 April 2020 9,945 6 View

Getting heterozygous band in Sanger sequencing in one direction only, not in the other direction, even after repetition. Why?

Even after repeating Sanger sequencing for a particular genetic region, heterozygous band peaks can be seen for a particular nucleotide position, only with one of the primers used for...

21 February 2020 5,209 1 View

Did anyone work with SKOV3 cells as in vitro model of Polycystic Ovarian Syndrome (PCOS)?

If anyone has used SKOV3 (ovarian cystadenocarcinoma cell line) as an in vitro system for Polycystic Ovarian Syndrome (PCOS), please share the journal reference and/or protocol used to establish...

07 August 2019 8,451 1 View

Has anyone faced difficulties recovering a radiolabeled product from a borosilicate glass vial after product dry down?

I have recently been working with Ga-68 labeled biomolecules and I have only 50% recovery from my borosilicate glass vial after the product has been dried at 50 degree C under a stream of nitrogen...

01 May 2019 319 3 View

AC or DC coupling: which one is best for Solar plus energy storage projects?

Energy storage system can be added to Solar plants through AC coupling or DC coupling. Which one is convenient and easy ?

18 March 2019 1,528 4 View

Different shear sense in a ductile shear zone?

Hi all, I am working on a ductile shear zone (E-W trending, near vertical to steeply dipping to the north) where the rock is dominantly mylonitic and stretching lineations are in most cases down...

15 January 2019 4,044 13 View

Do the performance in terms of life cycle, energy density vary with different shapes (cylindrical, pouch and prismat) of same lithium ion chemistry?

Generally, most of the research papers talk about the performances of a Li-ion half cell, coin cell or pouch cell. Do those performances are comparable with other shapes (cylindrical, prismatic)...

21 May 2018 4,172 5 View

How Can i measure concentration of a particular Compound in an metabolite using NMR? I am new to NMR Spectroscopy. Please give a detailed answer?

I need to measure Concentration of a compound in cell extract. How can i do it. thanking you

20 November 2017 6,221 3 View

How does polymer addition affect the conductivity of surfactant solutions ?

I am adding PHPA polymer to cationic and non-ionic surfactant solutions and found the conductivity values to decrease. I wish to know why does it behave as such?

11 September 2017 7,169 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View