complete map or description of the vector might help. also i am planing to do cloning and expression, so names of other vectors that are compatable( restriction sites Nco I and Not I) with this vector also i would like to know. thanks
I am doing HIC for purification of my protein. I am doing binding at 2 M ammonium sulfate and eluting at 1.4 M. Therefore,will lower concentration affect binding or elution of my protein
07 August 2019 5,117 7 View
i have few sets of small molecules which I want to test in BALB/c mice against Plasmodium berghei strain using artemisinin as a positive control. Also I was wondering whether artemisinin is...
05 June 2018 1,222 3 View
my vector size is 3.8 kb and gene size is 1191 bp. but after isolation of recombinant plasmid and doing double digestion with BamHI and NotI i am not getting proper results i.e. fallout should...
02 March 2018 7,321 9 View
I am new to this field and would like to screen chemical libraries with the help of auto dock.
08 September 2017 5,971 1 View
whether i should use normal autoclaved milliQ water or endotoxin free water or some buffer should be used for this work?
08 September 2017 216 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View