Prakhar Agrawal

6 Questions 9 Answers 0 Followers

Questions related from Prakhar Agrawal

Can protein binding to Phenyl Sepharose column be done at 1.6 M ammonium sulfate if protein is getting eluted at 1.4 M ?

I am doing HIC for purification of my protein. I am doing binding at 2 M ammonium sulfate and eluting at 1.4 M. Therefore,will lower concentration affect binding or elution of my protein

08 August 2019 5,118 7 View

How to determine concentration of test small molecules in mice?

i have few sets of small molecules which I want to test in BALB/c mice against Plasmodium berghei strain using artemisinin as a positive control. Also I was wondering whether artemisinin is...

06 June 2018 1,224 3 View

I have done double digestion after transformation but i am not getting right fallout?

my vector size is 3.8 kb and gene size is 1191 bp. but after isolation of recombinant plasmid and doing double digestion with BamHI and NotI i am not getting proper results i.e. fallout should...

03 March 2018 7,322 9 View

How to screen drug library with the help of auto dock?

I am new to this field and would like to screen chemical libraries with the help of auto dock.

09 September 2017 5,972 1 View

How to reconstitute heparin sodium salt powder ?

whether i should use normal autoclaved milliQ water or endotoxin free water or some buffer should be used for this work?

09 September 2017 218 1 View

Vector map of pRSF vector with TEV cleavage site to remove his tag?

complete map or description of the vector might help. also i am planing to do cloning and expression, so names of other vectors that are compatable( restriction sites Nco I and Not I) with this...

06 June 2017 4,774 0 View