Variability during successive rounds on random oligonucleotide library synthesis?

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Jun Lu

not sure. because the primer is suitable for all oligonucleotides. thus some oligonucleotides will not be duplicated.

Fawaz E. Alanazi

Yes,

After amplification, purification, the first PCR product will be used for the second round as a template. Before SELEX, check the quality of your library, do qPCR for at least 10 rounds to be sure it will work or not.

Good luck