Does anybody know the best stain for vaginal cytological evaluation of mouse estrous cycle?
People in my lab use toluidine blue
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...
21 May 2024 8,614 4 View
Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...
21 May 2024 2,171 0 View
Dear Experts I have optimized an organic molecule using DFT/B3lYP/ 6-311G using Gaussian16. However, after the optimization, it was found that some bonds were missing from the molecule's...
17 May 2024 9,828 1 View
I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...
12 May 2024 1,045 4 View
I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...
29 April 2024 8,231 1 View
What requirements (or qualities) are most important to be a good university professor?
22 April 2024 3,009 5 View
I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...
16 April 2024 2,051 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
What role do microorganisms play in the cycling of matter and role of microbes in the biogeochemical cycle?
25 July 2024 3,096 2 View
What is the exact mechanism of synthetic agents showed the cell cycle arrest in G0/G1 and G2/M2 phase in flow cytometry analysis.
28 June 2024 5,950 2 View
When I applied the cell cycle algorithm to these two samples in FlowJo, I encountered some concerns regarding the accuracy of the fit. The images include both a control and an apoptosis-inducing...
18 June 2024 1,984 0 View
For additional information, I am a master's student looking for a LCA software to do my thesis. So a less expensive one is my first priority, but if it is not able to help me with a clarity...
18 June 2024 8,376 3 View
I'm using flow cytometry with PI staining to analyze cell cycle arrest of Drosophila S2 cells after irradiation. Then, in every treatment including control (normal culture), the number of cells in...
12 June 2024 10,034 0 View
Some Other Questions, if you can answer: What types of data are essential for conducting an LCA, and where can I find reliable data sources for water treatment technologies? How do you deal with...
11 June 2024 4,791 0 View
Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...
03 June 2024 8,431 2 View