Using powder XRD data organic molecule, how to get bond lengths in angles of molecule?

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How to get recent research on poultry production?

poultry production in nigeria

29 July 2024 5,518 2 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What can I do if primer solution gives band in electrophoresis before PCR reaction?

Trouble 1: I ran a PCR reaction and found a low molecular weight band (100 bp) when doing electrophoresis. 1ng plasmid (OD260/280=1.84) was used as template. Final concentration of primers was...

13 June 2024 5,540 6 View

Please indicate whether you believe that the use of virtual reality has a positive or negative effect on people ?

Good morning, I am currently engaged in research in the field of virtual reality. I would be grateful if you could share your opinion on the subject. Your input is invaluable to me. Kind...

25 May 2024 8,567 5 View

Can bands of cell lysate be seen during SDS-PAGE?

I want to detect my target protein through WB and it worked well before. But now I could not get any signal for both my target and the GAPDH, so I ran SDS-PAGE. I expected many bands on the gel of...

23 May 2024 613 6 View

What is my problem when amplify a target gene from cDNA?

I want to copy a target gene from cDNA into a plasmid. The primers were designed according to the CDS sequence from NCBI. But when I performed PCR reactions I could not get any target bands. So...

15 April 2024 2,115 4 View

Can I incubate another secondary Ab after imaging with ECL during WB?

I found to use a wrong secondary Ab for my WB when I visualized it with ECL, can I wash with TBST for several times and incubate the right secondary Ab? Another question is why my bands are not...

16 February 2024 3,187 4 View

Qual è il ruolo dell’employer branding per una Employee Value Proposition di successo?

👋 Sto attualmente conducendo una ricerca finalizzata ad esplorare come le attività di employer branding possano permettere alle imprese di garantire una EVP (Employee Value Proposition) di...

17 January 2024 9,183 1 View

Questions about regulation of signal pathway through changing in protein expression level?

When I overexpressed a protein in a cell line, I found decrease of phospho-PLC (phospholipase C) at the protein level through WB. But when I knocked out the protein, there was no difference of...

10 January 2024 8,201 3 View

Did I use Cre correctly?

I want to use the Cre LoxP to knock out my target gene in a mammalian cell line. According to the mechanism, I need to add two loxP sequences at both ends of the gene through homologous...

21 November 2023 5,133 1 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

MDCI module in Orca software?

Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...

21 July 2024 1,632 0 View

What are the principles of physical chemistry?

what are the main points

10 July 2024 9,236 5 View

What is the recommended type of pseudopotentials for QE?

Hello dear colleagues and professors Please what is the recommended type of pseudopotentials for QE? ultrasoft or norm conserving?? And from where get all types of pseudos (us, nc, pbe,...

09 July 2024 9,541 5 View

Is there any guidelines about zooming out a chromatogram?

Hi, Just curious to know that in chromatography, a chromatogram should be zoomed to what scale? 10 times of output , 50X or ..? Is there any regulation or general chapter which describes this?...

03 July 2024 3,119 6 View

Antonio OfExova

I use expgui, and fullprof suite

Everton Bonturim

EXPGUI and Fullprof is good option. In this case, the A (lattice parameter) can be measurement (by Rietveld Refinement or Bragg's Law) and compared with the bond lengths for the molecule. You see in http://www.ccp14.ac.uk/

Imad-ud- Din

use expgui, and fullprof suite this is good option