Does anyone prefer this method over the traditional delta delta cT method? It seems that the LinReg method is more reliable than the delta delta cT method as it measures primer efficiency of each well, in each run. But it seems that the consensus is still with the traditional delta delta cT method. Just wondering if there is a reason for this as papers published on LinReg qPCR show strong evidence over its competitor?
I prefer this method, but up to now any of my thermalcyclers software supports this alternative approach. Must of us, in most cases, works with the standard sofware in order to no spend much time
http://link.springer.com/article/10.1007%2Fs12223-013-0255-5
best regards
Recently one our reviewer suggested to use LinReg to analyze qPCR as we had 3 groups to compare. I am not clear what is the advantage of this?
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