Who has experience with the new AriaMx Real time PCR machine from Agilent? While the machine itself seems to work well, the evaluation software is severely crippled and it contains a whole lot of errors.
We complained several times more than 5 months ago but the new software version 1.2. that came up recently not only did not repair anything but on top it became extremely unstable!
I am extremely disgusted that we as customers are used as beta-testers for an obviously flawed product.
Please see the attached file with a list of the most obvious problems and give me feedback about your experience with Agilent. Maybe if more users voice their concern in public, Agilent finally will listen.
Hi Robert,
I just saw your nice paper 'Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes'. Congrats!
About the Agilent Aria MX - I guess you try to multiplex PCR with the device - right? We have a Biomark fluidigm tool here in Lund. There software is a little better, but we nevertheless have developed a web software to analyze the resulting data.
If you are able to get a tab separated table from the Agilent Aria MX you can try to upload it to http://stemsysbio.bmc.lu.se/SCexV/files/upload/ and use the analysis pipeline there. If you do not mind sharing any data with me I can try to define a Agilent specific upload filter for you.
/Stefan
Dear Jessica,
the machine by itself works, the quirks of the software drive me crazy....If you have questions about protocols, just send me a private email and I'll see what I can do for you.
Best
Robert
Dear Jessica,
I also experienced some problems with the software when I started to use it, especially to try to understand the analysis of the data using the Pfaffl equation. The software's certainly not user friendly. I discussed my data with the Agilent technical team and then I decided to contact Prof. Bustin, one of the authors of the the MIQE guidelines, and that was inspirational.
I prefer to take the raw data and run every analysis in Excel, where I can check exactly where are the numbers coming from. I would suggest you to try to do the same. I am happy to send you a template of my excel file.
In terms of protocols, theremofisher has a superb handbook. I recommend it!
https://www.thermofisher.com/uk/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-handbook.html?icid=Default_WB21066
Kind regards,
Maria Teresa
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