Use of pooled samples in RNA-seq experiments. Can we have research gate community experience and opinion about this issue?

08 August 2013 0 9K Report

We're planning for the first time a RNA-seq experiment, hoping to identify differentially expressed genes in a non-model mussel (de novo assembly). We have some doubts on using or not pooled samples to reduce biological variation (like 3 pooled samples as each of 3 biological replicates per treatment). Can someone please give some light on this issue?

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