Guilherme Toledo

3 Questions 4 Answers 0 Followers

Questions related from Guilherme Toledo

Use of pooled samples in RNA-seq experiments. Can we have research gate community experience and opinion about this issue?

We're planning for the first time a RNA-seq experiment, hoping to identify differentially expressed genes in a non-model mussel (de novo assembly). We have some doubts on using or not pooled...

08 August 2013 9,047 0 View

What kind of evolutive questions can be resolved with RNA-seq experiments?

I have two RNA-seq experiments (two different but related species) and want to use more of the data, with focus on evolution based questions. I have some genomes of the same group available for...

04 April 2013 9,810 3 View

How much data is needed for analysis of differential expression using RNA-seq data without genome/transcriptome reference?

I read in seqanswers something about 4-5 Gb of paired-end data for each replicate to assure some depth. Any help from people with some experience in this kind of analysis is appreciated.

04 April 2013 5,818 8 View