dear all,

I am trying to follow the plasma membrane of Jurkat cells using DiBac43 and a spectrofluorimeter. But when I stimulated the cells with phytohemagglutinin, known to hyperpolarize the cells, I got an increase of DiBac43 fluorescence, meaning a cell depolarisation...

The protocol I used is: Jurkat cells + 250 nM DiBac43 during 15 min for loading, and after direct measure, still in presence of extracellular 250 nM DiBaC43