Dear all,
I am looking to do FACS sorting from a copopulation of BV2 and RAW 264.7 cells back to individual populations after LPS or alpha-synuclein challenge. I understand that they are both macrophage-derived cells and am having issues finding a unique expression marker for each population. Do you have any good strategy for FACS sorting for the cells?
Many thanks and kind regards,
You may consider transfecting one of the populations with GFP before the co-culture to allow for FACS discrimination following your treatments. Unactivated BV-2 cells probably express detectable levels of P2Ry12, which I wouldn't expect in the RAW cells (though you would expect down regulation with an LPS challenge). As you indicate, most of the markers found on the microglial like cells will also be expressed on the RAW macrophages.
Good luck!
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