I have prepared DNase I recombinant (3000 U/ml in 50 mM Tris-HCl, pH 7.5, 1 mg/ml BSA) from DNase I recombinant 10000 UNITS and used it as a positive control in TUNEL Assay but it has not worked (No signal on positive control showed). I am wondering if I prepared it correctly.

Would you mind getting me the correct calculation to prepare the required amount from the DNase I recombinant 10000 UNIT, please?

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