Hi
I am using a BioRad PDS-1000 /He system (equipped with
1,350 psi Rupture Disks #1652330) for biolistic transformation of plant embyos.
I am noticing that most of the time the rupture disks do not pop, but rather they get separated in two layers, even if after reaching the suggested vacuum (28 mm Hg) and 1350 psi when pressing the "fire" button.
Is anyone experiencing similar difficulties?
Thanks
Dario