I am doing some seahorse assays with bone marrow derived macrophages and I see no reponse to oligomycin. During an ATP rate assay, OCR is not decreasing and ECAR is not increasing. Any thoughts about this?
Hello! Were you able to solve it? If not, please detail the protocol you are doing. There could be several reasons.
I am trying to simulate compression test with loading rate as 2mm/min for 20mm displacement. By creating time vs displacement table eg., (0,0) (0.1,-2) (0.2,-4) ...... (1,-20) , is it the way...
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I'm in need of global land cover data from 1980's to recent (flexible with the mentioned years) in 0.5 degree resolution for representing the data in our project. I have browsed through several...
06 January 2019 3,010 7 View
I am working on some new composition where I want to find the compound is explosive or not.I am using TG-DSC but from that how can I predict the composition is explosive or not?
06 March 2017 1,956 3 View
Latex used in concrete is Latex SBR(Styrene Butadiene Rubber)
07 March 2016 5,828 1 View
I am trying to design basic horn antenna but when merging the solids I got an error saying "ACIS error in boolean operation". Whatever I do in the design, while merging I am getting this error....
09 December 2015 9,837 8 View
I am not sure whether the question i am putting forward is right one.. if the question is right or wrong i may learn from both... kindly clarify these questions.. 1.is it posiible to design...
01 January 1970 8,562 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Hai, Iam doing experiment about novel compound to induce thermogenesis on 3t3-L1 cells. The beta actin has been adjusted, but when i did western blot and imaging with image J, the result it is...
14 July 2024 3,354 1 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View