What do you think about the effects of climate change, global warming and decreased drought frequency on agricultural employment?

Most chimate and agricultural research has focus on yield or crops, not the people who workers labour outside. I wonder that, when expose to extreme heat exposure in the future, who will produce...

27 July 2020 2,677 12 View

Why MDA-MB-231 cell line die after passage ?

Hello All, I'm working with MDA-MB-231 cell line and I always perform same procedure while I'm passaging these cells. After unfreeze the cells ( at first I freeze them with 5% DMSO, 95% FBS...

21 November 2018 4,728 12 View

I confused about buffer exchange. which method is useful ?

I have a DNA solution in 0.3 M K2HPO4 and I want to remove phosphate dibasic solution and resuspended DNA in water or TE buffer. How can I do it?

16 November 2017 7,499 3 View

How can I seperate HDL(high density lipoprotein) subclasses(like HDL 2a, 2b, 3a, 3b, 3c)by ultracentrifugation?

I seperated lipoproteins(HDL, LDL, VLDL) by ultracentrifugation but I can not progress. I have problem about g(RCF) and hour for ultracentrifuge to seperate HDL subclasses. I followed a article...

27 March 2017 4,391 7 View

Which steps to follow second-order formative construct (Type 2)?

Hello everyone, I have one second order formative construct in Type 2 which consists of 3 sub-constructs and 4 reflective constructs. Basically the model is as attached. The question is, I have...

16 February 2017 7,479 3 View

Hi,Can anyone suggest precipitation method for casein protein from milk,please?

26 October 2016 2,852 12 View

How can I dissolve the tantalate (Cs, La, Ta) remaining at the bottom of my glassware?

15 March 2016 5,583 2 View

Is there a chemical inhibitor of Dicer?

We need to inhibit ribonuclease Dicer to see whether miRNAs are functional. Thanks in advance..

16 March 2015 1,023 3 View

Antioxidant activity measurement in fresh vegetables

Are there anyone using FRAP, DPPH or ABTS assay for measuring antioxidant activity in fresh vegetables? Or do you have any suggestions about more practical,reliable methods? thanks

25 January 2012 5,749 18 View

How a motivation can be provided to keep Y and Z generation in agricultural sector?

The average age of agricultural enterprises is growing. For example; in the United States, the average age of farmers is 58, in Japan 67, and in Africa 60. Young people don't tend to stay in...

01 January 1970 5,402 19 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

What is the Greedy windowed algorithm description?

Hi Dear Ph. D., I have carefully read your paper on anomaly detection in smart meters. Unfortunately, the research does not decribe the greedy windowed algorithm, neither its complexity, nor the...

10 February 2021 2,462 3 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

How to incorporate Monte Carlo simulation in for a real trading data?

Hello, My area of research is to valuate Exchange option (Margrabe option). I am looking for Monte Carlo simulation technique that can be applied to some real trading data. I have evaluated them...

05 February 2021 1,605 4 View