My separating gel is not getting polymerized. (It does not set). I am following protocol by Schagger, Von Jagow protocol. Can anyone please forward me your protocol for Tricine SDS PAGE?
Suggestions given by others should solve your problem but in case those do not work you can increase the amount of APS and TEMED. You should also check the TEMED whether it is too old or freshly procured. For an alternative protocol for Tris-Tricine PAGE, you can consult the protocol given in Chapter 10 in Current Protocols in Molecular Biology by Ausubel.