01 January 1970 0 3K Report

La causa de que cada vez haya más niños dentro del Espectro Autista, ¿es una cuestión de origen genético o ambiental?

¿En qué medida uno u otro?

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FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View

Dyslexia and Virtual Reality yes or no?

Do you think the use of virtual reality with dyslexia students is good?

23 June 2024 8,437 2 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

If I am doing a thematic analysis based on one group, is it okay to add insights from another group of interviewees?

I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...

21 June 2024 3,305 6 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

19 June 2024 2,125 3 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

Is it okay to structure the results section of a thematic analysis based on your research questions?

I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...

16 June 2024 1,116 3 View

How can I add my old article to my profile, if I am the first author, but I have different name before marriage?

Please note, that this is my first manuscript under the name Mária Čápová. Then I have been married, and my name change to Plachá, but I would like to have this article in my profile. Ontogenetic...

16 June 2024 7,178 0 View

Can I design a probe for taqman qpcr in antisense?

I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...

16 June 2024 1,506 1 View

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What's the Molecular weight of siRNA?

Hi guys, I have 27 mer siRNA duplexes from Origene, and the company didn't provide the Mwt. I tried to search but no luck so far How can I calulate it or find it?

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Source intensity for beam source MCNP6.2?

I am trying to determine the most appropriate way to define a beam source of a given intensity. Normally one define their beam intensity via an FM card scaling to power but I am planning to use my...

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Myc tag antibody ?

Hi All I am recently doing WB fron transfected cells HEK293(using a plasmid tagged by Myc & DDK) For protein extraction i used RIP buffer and protease inhibitor.The Myc antibody i sued from...

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What is the acceptable error range when validating multibody systems?

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¿Por qué se ha utilizado la futurología para comunicar la ciencia y tecnología?

La descripción de escenarios del futuro para comunicar los avances científicos y tecnológicos contemporáneos en publicaciones de alcance popular es una práctica antigua, cuyo origen histórico...

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I want to make a gene overexpressing cell line?

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Use of 3'utr plasmids with luciferase as reporter and RFP as control?

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Looking for Myc or Tags-T2A-PURO/Blast vectors?

Hi all, I am looking for a vectors backbone containing an expression cassette for epitope tagging my candidates are -Puro/blast-T2A-3MYC (other tag will do ) -MYC-T2A-PURO/Blast (other tag will...

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