Transformed bacteria grow in antibiotic plaque but do not express the desired protein, how can this be?

More Yaiza Codeseira's questions See All

DGGE. Does anyone have had problems with the separation of fungal bands on acrylamide gels?

24 October 2018 8,251 6 View

Has anyone experienced problems with BrdU staining the cytoplasm instead of the nucleus?

I'm doing BrdU staining in a cell line. I've treated the cells with 1.5 ug/ml of BrdU for 1h. Then I fixed them with 2% PFA for 15min, denaturated with HCl 4N for 10min RT, washes in PBS to...

28 September 2015 5,989 8 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

01 February 2021 3,735 3 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How to compute the phase from a fringe pattern using Hilbert Huang Transform in MATLAB?

I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...

10 January 2021 5,036 1 View

Which statistical test is best to run on a heavily right-skewed dependent variable?

My dependent variable when graphed is heavily right-skewed. All attempts to transform it has failed (Log, Log10, Sqrt, ..). The most ideal test I would like to run is a multiple linear...

07 January 2021 2,705 4 View

How can I estimate and plot Logarithmic Error Bars (in Excel, preferably)?

Dear Dr.colleagues, I am currently plotting the dynamics of an infection in Excel. For each sampling day, I plotted the mean infection load of the population, using the standard error for the...

07 January 2021 2,069 3 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

How i can fix this problem for python jupyter" Unable to allocate 10.4 GiB for an array with shape (50000, 223369) and data type int8"?

my code: #building tf-idf from sklearn.feature_extraction.text import TfidfVectorizer vectorizer = TfidfVectorizer(analyzer = message_cleaning) #X = vectorizer.fit_transform(corpus) X =...

28 December 2020 3,624 2 View

Hassan Nasser

Dear Yaiza you can read this articles, i hope it will help you:

https://worldwidescience.org/topicpages/p/plasmid+dna+fluorescence.html

You can also see this revolution project:

https://scitechdaily.com/major-scientific-advance-deepmind-ai-alphafold-solves-50-year-old-grand-challenge-of-protein-structure-prediction/