Hello,
Lately I have been attempting to standardize a method for Ru detection (349.9 nm) in digested cell samples. Everything seemed to have been going smoothly until yesterday.
Of several series of standard additions I noticed one sample along with each of its additions had a distinct background abs. compared to the remaining samples. A Ru HLC (lamp) was used. Deuterium (D2) background correction was used due to availability. Note that each series is similar to the other in matrix composition (i.e., cell digestate, PBS, HNO3). Both the Ru HLC and graphite furnace are well within their expected lifetimes.
In one series, the background abs. was on average -0.04 AU. For the others, it was closer to +0.22. What could cause this irregularity or drop in intensity?
A typical 'Deuterium Lamp' emits a continuous and decreasing spectrum from 160-400 nm.
That I do not know. I have seen references conflict over the lamp's useful range but as for the instrument's parameters, that is not one explicitly stated nor obvious by spectral output since it is Abs. Intensity Vs. Time (s).
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