Assuming the assay is done properly, why would values be 110-125%? The one thought I had is that different cmpds may have higher response factors. Samples were fractions from a crude extract.
The lab may be only using a single point for calibration and extrapolating. There may be something in the math.
When you are asking a question put yourself on the place of a potential commenter. What is your assay? How did you calibrate it? How did you obtain125%?
Mr. Kelm,
Your outcome over 100 % is a result from the error contribution, due to method and methodology.
Exact quantification, currently, is carried out only by mass spectrometry, however, within the framework of our innovative own-authored (to me and my co-author's theory according to the authorship shown, below) strochastic dynamic theory and model formulas. The method has been tested, so far, on mixtures of analytes at concentration level up to pg.(mL)-1, showing coefficient of linear correlation between theory and experiment /r/ = 0.99997 - 1.
Please, conside detail on references [1,2].
[1] Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller
Analytical Chemistry Letters, 10 (2020) 703-721; Received 13 Oct 2020, Accepted 16 Dec 2020, Published online: 28 Jan 2021
Download citation https://doi.org/10.1080/22297928.2020.1865834
[2] Steroids, 164 (2020) 108750
Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller
Hi Mark Kelm
There are several analytical methods for "Total Phenolics".
Depend on your study and use of your data but your results can still be acceptable and useful.
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