first question
the specification for the microbial analysis of gelatin analysis in pharmacopoeia is the absence of E. coli and Salmonella. But when I test the product I cannot find E. coli, but I find facial coliform which is not required in pharmacopoeia (gas formation at 44 ° C). Is the product acceptable or unacceptable due to presence of facial coliform although it is not required?
second question
It was found that the total microbial count of the gelatin product is reduced after a period of production and storage at high temperature. If we test the product and we find that it contains Escherichia coli, and if we store the product for long time then test
Dear Salah
You have to follow standard specifications of products to accept or refuse the tested product.
And You have to follow accredited standard steps for counting or detection microbiological tests of products.
Regards
agree with Mr. Nasr, it is necessary to follow all standard specifications prescribed for the product, after testing with standard procedures.
Fecal coliform bacteria ( FC ) are a subgroup of the total coliform bacteria that can be found in the intestines and feces of warm blooded animals ( humans being , pigs, dogs, cows etc ) The presence of thermotolerant coliforms nearly always icdicates fecal contamination.The product is unacceptable.
As regard for the first question the result is acceptable as followed in pharmacopoeia.
for the second question presence of coliform group after following the standard procedures is unacceptable.
1. The presence of faecal coliform in any product is unacceptable.
2. Some bacteria including E.coli can enter into a state of being viable but non culturable. Find other ways of testing to be sure that the microbes are truly killed.
it is important to follow all standard specifications prescribed for the product.
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