To who this may concern ,

I am trying to stain cross-section of a nerve with toluidine blue for morphometric analysis of the section. I am trying to identify the fibre diameters and general characteristics. I am using 5-6 micrometer sections which have been embedded in paraffin. I have not been able to get a good technique for the stain due to my sections not retaining the blue color. In other words, my sections turn pink/purple after staining and I am not able to analyze the tissue as the color is very faint. Any suggestions or protocols for this stain ?

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