florescence intensity of my iadeans labelled protein changes from 200k AU to 250k AU under two different conditions. whether this much change in the intensity is considered to be significant??
Hi there,
The fluorescence intensity modification has to be normalized towards the full amplitude of fluorescence modification to conclude on its significance
It might be a statistically significant difference if, upon making multiple measurements and performing a t-test, the P-value is below the desired value (usually 0.05).
However, the statistical significance does not address whether the difference indicates a meaningful effect. The change in the conditions may alter the EDANS fluorescence intensity without indicating a conformational change in the protein. For example, fluorescence intensities of fluorophores in water usually decrease as the temperature increases. So you should test to see whether the change of conditions also affects the fluorescence intensity of EDANS that is not attached to a protein.
as basic information- we can not know, what is signifcant, if we got just this little information you gave. To say provokant- without knowing, the background intensity, and the conditions, we could even say, you do not see significant fluorescence. Its because youre scale is relative.
thanks all for the suggestion..@Adam , yes i will check for change in intensity for the EDANS that is not attached to the protein..
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