To study inflammation activity by pro inflammatory response, will the huvecs be the choice and in which passage is most optimum to do the study?

More Amierul Sharudin's questions See All

How to Solve the Error of "The elements contained in element set ErrElemExcessDistortion-Step1 have distorted excessively." on ABAQUS CAE?

I'm currently trying to run a ballistic impact simulation on Kevlar composite plates on ABAQUS CAE through the dynamic explicit simulation. However, each time I try to run my simulations, this...

02 May 2021 589 3 View

Possible process in optimizing LPS as inducer?

what are the dosage?

11 April 2016 9,970 1 View

Does anyone have any references regarding aqueous extraction protocols?

i need to understand of how the extracts will be carried out for treatment purposed e.g 200g of fruits soaks all night and later boiled in 5L of distilled water.

08 May 2015 6,765 6 View

Does anyone have the data sheet for heparin (17513-54) from Nacalai Tesque?

17513-54 from Nacalai Tesque

04 January 2015 2,135 0 View

Where can I get a detailed explanation on the population doubling level (PDL) by HUVECs?

How do you calculate PDL? I need some expertise on this issue along with journal or article regarding population doubling for huvecs.

20 November 2014 6,738 12 View

What are the most effective protocols to measure the tissue factor activities?

I need help from the experts to choose the reasonable protocols/methods on screening/evaluating/measure the tissue factors (TF) activities.

05 October 2014 3,090 2 View

What is the most efficient serum to supplement on cell cultured for inflammatory study? Any differences to apply the media between HUVECs or HAEC?

Which is more relevant HUVEC or HAEC? Efficient media for inflammatory study?

19 February 2014 1,070 3 View

How many passage is ideal to use for cell culture and how to make the commercialized culture inflamed? what is the best media?

I would like to do a cellular level screening of the pro inflammatory activities. Any suggestion for reading, for a detail protocol and procedure?

18 February 2014 5,999 2 View

How to decide/select the best inflammation mediators activated from vascular endothelia cell?

Im confused due to different events that will activate different mediators. For example, plaque formation, inflammation and coagulation generally activate different mediators.

08 February 2014 797 2 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

Does the bread-making process lead to a sufficient reduction in lectin activity of bean flour to guarantee food safety?

I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...

17 July 2024 7,293 3 View

What is homomenthyl cinnamate?

the molecule name is citated in old literature about organic sunscreen molecules

10 July 2024 5,576 2 View

Interactions between oral microbiota and oral sensory systems: impact on oral comfort and pain perception?

This research question explores the complex interactions between oral microbes and the oral sensory system. The diversity and function of oral microbial communities play an important role in oral...

09 July 2024 3,601 0 View

Any better protocol for entrapment efficiency of curcumin?

I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...

01 July 2024 7,007 1 View

What causes unexplained rash on the whole body after consuming Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor, Chela-fer and Myoprin?

I want to understand what are the contraindications and side effects caused in diabetic patients who are on chronic medication (Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor,...

25 June 2024 984 0 View

Hello, currently I am working to develop biopesticide product use Metarhizium. I would like to know how to formulate it so it can be effective?

Currently, I am working in oil palm plantation industry. This Metarhizium will be used to control the population of pest Oryctes rhinoceros. I need clue to formulate Metarhizium so it can be...

24 June 2024 9,926 3 View

I need to buy reducing agent Bruggolite FF6 M, how can I buy?

I have searched for CAS no. but cannot find.

24 June 2024 2,737 2 View

Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

What is the potential role of phytochemicals in the prevention and management of asthma symptoms?

Asthma is a chronic respiratory condition marked by inflammation and narrowing of the airways. While traditional treatments involve medications like corticosteroids and bronchodilators, there's...

14 June 2024 8,063 2 View

Subhadeep Chakrabarti

Passage 1 (initial isolation is taken as passage 0) to passage 3 are ideal. Passage 4 can be OK, anything beyond that is likely to show diminished inflammatory response and altered morphology.

Amierul Sharudin

Tq Dr. Are there any journal mentioning about the optimum passage to work on inflammatory response on HUVECs?

Vincenzo Brancaleone

Passage 1 or 2 would be the best option, however as already mentioned you mya get to passage 3, but not beyond.