If you have a light microscope with eyepieces that can be exchanged, acquire an eyepiece with built-in grid for measuring specimens. You do not need a projection microscope to use it. You can also purchase lower-cost glass slides that have a grid etched on their surface and you can use the grid to measure diameter of fibers. Be careful with your calculations on the magnification.
Remember that the diameter of a wool fibre can change significantly depending on the relative humidity as wool fibres swell when they absorb moisture. That is why commercial wool testing laboratories measure fibre diameter after a period of 'conditioning' at 20 degrees celsius and 65% relative humidity. For consistent measurements it is important to ensure that the environment in which you are measuring the samples, does not vary too much in temperature or humidity - particularly if you are measuring a large number of samples over a period of days or weeks. If this is the case, it may be necessary to select a number of 'control' samples (n = 5) of various diameters and measure these at both the start and end of each day and use any changes over time to 'correct' your measurements for variation in environmental conditions.
Thank you for reminding me the measurement conditions, I'll take your comments into consideration during treatment. I would be very interested if you can give me references or wool discussing authors thank you in advance for your cooperation. Best regards
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