May I know is there anyone who have experiences on infants (0-4 months of age) saliva collection and analysis who can share? I am wondering what factors should I consider before I choose which kits to go for? Do I really need to use a kit? PM or comment are welcome.
You may read Strategies for salivary cortisol collection and analysis in research with children K Hanrahan, AM McCarthy, C Kleiber… - Applied Nursing …, 2006 - Elsevier
for ethical strategies to do this.
we have done some research on cortisol in saliva, using salivette collection tools. Be aware that there are techniques to use a 'saliva' induction technique, but a higher saliva flow will result in differences in estimates. Finally, you should compare to 'free' plasma' concentrations, and not the total concentration. There is published experience with caffeine in saliva in neonates also (check de wildt).
ref on our experience with cortisol in saliva:
Newborn genome-wide DNA methylation in association with pregnancy anxiety reveals a potential role for GABBR1. Vangeel EB, Pishva E, Hompes T, van den Hove D, Lambrechts D, Allegaert K, Freson K, Izzi B, Claes S.
Clin Epigenetics. 2017 Oct 3;9:107.
Hi Karel, thanks for the answer, may I know did you use Protease Inhibitor Cocktail in your samples?
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